Initiation of Protein Synthesis in Saccharomyces cerevisiae Mitochondria without Formylation of the Initiator tRNA

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Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA.

Protein synthesis in eukaryotic organelles such as mitochondria and chloroplasts is widely believed to require a formylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation. Here we show that initiation of protein synthesis in yeast mitochondria can occur without formylation of the initiator methionyl-tRNA (Met-tRNA(fMet)). The formylation reaction is catalyzed by methionyl-tRNA formyl...

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The effect of N’“-formyl-H,folate on mitochondrial peptide chain initiation has been studied in isolated mitochondria of Saccharomyces cerevisiae. The addition of N”‘-formyl-H,folate strongly stimulates the incorporation of amino acids into mitochondrial protein at both 6 and 15 mu Mg2+. Still higher stimulation (up to IO-fold) has been obtained in the production of de nouo synthesized initial ...

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Formylation of initiator tRNA methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression.

Eucaryotic and procaryotic organisms differ in two aspects of their translation machinery: polycistronic messengers are expressed as a sequence of individual proteins only in procaryotes, and the initiation of protein synthesis proceeds with an initiator tRNA which is found to be modified (formylated) in procaryotes and not in eucaryotes. In the present study, we show that formylation is requir...

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Dependence of mitochondrial protein synthesis initiation on formylation of the initiator methionyl-tRNAf.

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More surprises in translation: initiation without the initiator tRNA.

O of the most widespread beliefs in molecular biology is that protein synthesis is initiated with methionine or formylmethionine in all organisms, by using AUG as the initiation codon and a special methionine tRNA called the initiator tRNA (1, 2). Eubacteria, mitochondria, and chloroplasts initiate protein synthesis with formylmethionine, whereas archaebacteria and eukaryotic cytoplasm initiate...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 2000

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.182.10.2886-2892.2000